Crossing Gene Sets from GlyGen, GTEx, MoTrPAC and RummaGEO Reveals Nicotinamide as a Potential Modulator of Glycosylation, Aging and Exercise

Abstract

Biomedical omics resources are often treated as separate collections, which fragments insight and limits the ability to see shared mechanisms across diverse biological contexts. By crossing gene sets from four Common Fund librariesGlyGen Glycosylated Proteins, GTEx TissueSpecific Aging Signatures, MoTrPAC Rat Endurance Exercise Training Transcriptomics, and RummaGEO Drug Perturbation Signatureswe sought to uncover genes that are present in every dataset, reasoning that such maximal overlap highlights robust, reproducible signals. The GlyGen set contributed extracellular glycoproteins whose carbohydrate modifications influence matrix stability and innate immune clearance, while the GTEx aging signature captured blood expression changes associated with advancing age. The MoTrPAC exercise transcriptome added genes responsive to endurance training, reflecting physiological adaptation, and the RummaGEO nicotinamide perturbation signature intro- duced a drugrelated component that aligns with the observed pathways. The intersecting ten genesAMBP, APCS, APOC4, APOH, C8A, FGA, FGB, FGG, HRG and SERPINA6are all extracellular proteins central to hemosta- sis, complement activation and inflammatory regulation, and enrichment analyses consistently pointed to coagulation, fibrinolysis, platelet function and nuclear receptor transcriptional programs. Together these findings illustrate how integrating multiple Common Fund datasets can reveal a coherent biological module and suggest relevance of the nicotinamideassociated perturbation to coagulationrelated and inflammatory processes.